A lactococcal bicistronic vector system for gene delivery and method of preparation thereof
Nurulfiza Mat Isa. (2013) A lactococcal bicistronic vector system for gene delivery and method of preparation thereof.
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The present invention provides a bicistronic vector system based on Lactococcus lactis plasmid DNA into which an eukaryotic promoter, a first gene to be transcribed by the promoter, internal ribosome entry (IRES), a second gene to be transcribed by IRES and transcription terminator signal are incorporated in said order downstream of each other. The invention also provides a method of preparation of such vector.
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