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Purified T1 lipase, a novel construct of T1 lipase and methods thereoF

Raja Noor Zaliha Raja Abd. Rahman. (2011) Purified T1 lipase, a novel construct of T1 lipase and methods thereoF.

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Abstract

The present invention relates to method to substitute second step affinity chromatography with ion exchange chromatography by site-directed mutagenesis at downstream region of GST sequences (H215R and G213R). This is to enable to diferentiate the isoelectric point (pI) value of GST from the pI value of matured T1 lipase. Also, by obtaining a new construct, new T1 lipase from the above methods, the new construct and T1 lipase was purified using two chromatography steps affinity and ion exchange chromatography. Thus, as mentioned above, the said method provides an advantage to limit or eliminate the second affinity chromatography step.

Item Type:Patent
Keyword:Lipase; T1 lipase; Chromatography
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Application Number:PI2011700160
Filing Date:2011-11-09
ID Code:33123
Deposited By: Suzila Mohamad Kasim
Deposited On:23 Dec 2014 16:17
Last Modified:27 Sep 2016 09:30

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