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A method for detecting candida species in a specimen by polymerase chain reaction.

Chong Pei Pei. (2011) A method for detecting candida species in a specimen by polymerase chain reaction.

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Abstract

The present invention relates to a method for detecting Candida species in a specimen, comprising the steps of extracting nucleic acid from the specimen; performing polymerase chain reaction on the extracted nucleic acid using DNA primers for amplification of targeted nucleic acid sequence; detecting the amplified nucleic acid for each Candida species; characterized in that, in a single reaction, the DNA primers consist of: SEQ ID NO. 1 and SEQ ID NO. 2 or their complements for amplifying nucleic acid from Candida albicans; SEQ ID NO.3 and SEQ ID NO. 4 or their complements for amplifying nucleic acid from Candida glabrata; SEQ ID NO. 5 and SEQ ID NO. 6 or their complements for amplifying nucleic acid from Candida parapsilosis; SEQ ID NO. 7 and SEQ ID NO. 8 or their complements for amplifying nucleic acid from Candida tropicalis.

Item Type:Patent
Keyword:Candida species; Polymerase chain reaction; DNA primers.
Faculty or Institute:Faculty of Medicine and Health Science
Application Number:PI2011000631
Filing Date:2011-02-11
ID Code:32082
Deposited By: Suzila Mohamad Kasim
Deposited On:15 Oct 2014 16:18
Last Modified:15 Oct 2014 16:18

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