Purification of antibodies against hepatitis B.
Ling Tau Chuan. (2010) Purification of antibodies against hepatitis B.
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The present invention discloses a method for the purification of polyclonal immunoglobin G (IgG) against type B hepatitis infections, the method comprising the steps of: (i) adsorption of serum on diethylaminoethyl (DEAE) sepharose without elution, in which albumin content in the serum is removed: and (ii) adsorption of the albumin depleted serum on an immobilized dye followed by elution, in which the adsorbed antibodies are eluted in pure form.
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