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Optimization of extraction of novel pectinase enzyme discovered in red pitaya (Hylocereus polyrhizus) peel


Citation

Ahmad Zohdi, Nor Khanani and Amid, Mehrnoush (2013) Optimization of extraction of novel pectinase enzyme discovered in red pitaya (Hylocereus polyrhizus) peel. Molecules, 18 (11). pp. 14366-14380. ISSN 1420-3049

Abstract / Synopsis

Plant peels could be a potential source of novel pectinases for use in various industrial applications due to their broad substrate specificity with high stability under extreme conditions. Therefore, the extraction conditions of a novel pectinase enzyme from pitaya peel was optimized in this study. The effect of extraction variables, namely buffer to sample ratio (2:1 to 8:1, X1), extraction temperature (−15 to +25 °C, X2) and buffer pH (4.0 to 12.0, X3) on specific activity, temperature stability, storage stability and surfactant agent stability of pectinase from pitaya peel was investigated. The study demonstrated that the optimum conditions for the extraction of pectinase from pitaya sources could improve the enzymatic characteristics of the enzyme and protect its activity and stability during the extraction procedure. The optimum extraction conditions cause the pectinase to achieve high specific activity (15.31 U/mg), temperature stability (78%), storage stability (88%) and surfactant agent stability (83%). The most desirable conditions to achieve the highest activity and stability of pectinase enzyme from pitaya peel were the use of 5:1 buffer to sample ratio at 5 °C and pH 8.0.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
DOI Number: 10.3390/molecules181114366
Publisher: MDPI
Keywords: Fruit enzyme; Specific activity; Temperature stability; Storage stability; Surfactant agent stability
Depositing User: Umikalthom Abdullah
Date Deposited: 07 Apr 2015 08:15
Last Modified: 23 Dec 2016 16:09
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules181114366
URI: http://psasir.upm.edu.my/id/eprint/30827
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