UPM Institutional Repository

Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor


Citation

Masoumi, Hamid Reza Fard and Basri, Mahiran and Kassim, Anuar and Abdullah, Dzulkefly Kuang and Abdollahi, Yadollah and Abd Gani, Siti Salwa and Rezaee, Malahat (2013) Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor. The Scientific World Journal, 2013. art. no. 962083. pp. 1-9. ISSN 2356-6140; ESSN: 1537-744X

Abstract

Lipase-catalyzed production of triethanolamine-based esterquat by esterification of oleic acid (OA) with triethanolamine (TEA) in n-hexane was performed in 2 L stirred-tank reactor. A set of experiments was designed by central composite design to process modeling and statistically evaluate the findings. Five independent process variables, including enzyme amount, reaction time, reaction temperature, substrates molar ratio of OA to TEA, and agitation speed, were studied under the given conditions designed by Design Expert software. Experimental data were examined for normality test before data processing stage and skewness and kurtosis indices were determined. The mathematical model developed was found to be adequate and statistically accurate to predict the optimum conversion of product. Response surface methodology with central composite design gave the best performance in this study, and the methodology as a whole has been proven to be adequate for the design and optimization of the enzymatic process.


Download File

[img]
Preview
PDF (Abstract)
Statistical optimization of process parameters for lipase.pdf

Download (84kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Science
Institute of Advanced Technology
Institute of Bioscience
DOI Number: https://doi.org/10.1155/2013/962083
Publisher: Hindawi Publishing Corporation
Keywords: Optimization; Triacylglycerol lipase; Bioreactor.
Depositing User: Umikalthom Abdullah
Date Deposited: 10 Sep 2014 04:11
Last Modified: 08 Oct 2015 07:53
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2013/962083
URI: http://psasir.upm.edu.my/id/eprint/30240
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item