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Polymerase chain reaction detection of C. pseudotuberculosis in the brain of mice following oral inoculation

Abdullah, Faez Firdaus Jesse and Adamu, Lawan and Osman, Abdinasir Yusuf and Muhdi, Ahmad Fauzan and Haron, Abdul Wahid and Saharee, Abdul Aziz and Saad, Mohd Zamri and Omar, Abdul Rahman (2013) Polymerase chain reaction detection of C. pseudotuberculosis in the brain of mice following oral inoculation. International Journal of Animal and Veterinary Advances, 5 (1). pp. 29-33. ISSN 2041-2894; ESSN: 2041-2908

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Abstract

The aim of present study was to detect the presence of C. pseudotuberculosis in the brain of the mice following oral inoculation as a model using PCR. Caseous lymphadenitis is a chronic and subclinical disease of sheep and goats which has universal distributions, presenting enormous animal and flock prevalence. Total of 16 mice were used for this study, 8 mice were inoculated orally with 1.0 mL sterile phosphate buffered saline pH 7, while another 8 mice were inoculated with 1.0 mL of 109 colony forming unit of C. pseudotuberculosis. Seven different organs were collected during post mortem for the detection of C. pseudotuberculosis The result indicated 3 positive samples in lymph nodes, 5 in the brain and 1 in the liver. The PCR used in the present study may successfully be applied for the detection and diagnosis of C. pseudotuberculosis in the brain of the mice following oral inoculation.

Item Type:Article
Keyword:Brain; C. pseudotuberculosis; Detection; Mice; Oral inoculation; PCR
Faculty or Institute:Faculty of Veterinary Medicine
Institute of Bioscience
Publisher:Maxwell Science Organization
ID Code:29843
Deposited By: Nabilah Mustapa
Deposited On:04 Jul 2015 08:27
Last Modified:11 Feb 2016 14:10

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