Simple Search:

Polymerase chain reaction detection of C. pseudotuberculosis in the brain of mice following oral inoculation


Citation

Abdullah, Faez Firdaus Jesse and Adamu, Lawan and Osman, Abdinasir Yusuf and Muhdi, Ahmad Fauzan and Haron, Abdul Wahid and Saharee, Abdul Aziz and Saad, Mohd Zamri and Omar, Abdul Rahman (2013) Polymerase chain reaction detection of C. pseudotuberculosis in the brain of mice following oral inoculation. International Journal of Animal and Veterinary Advances, 5 (1). pp. 29-33. ISSN 2041-2894; ESSN: 2041-2908

Abstract / Synopsis

The aim of present study was to detect the presence of C. pseudotuberculosis in the brain of the mice following oral inoculation as a model using PCR. Caseous lymphadenitis is a chronic and subclinical disease of sheep and goats which has universal distributions, presenting enormous animal and flock prevalence. Total of 16 mice were used for this study, 8 mice were inoculated orally with 1.0 mL sterile phosphate buffered saline pH 7, while another 8 mice were inoculated with 1.0 mL of 109 colony forming unit of C. pseudotuberculosis. Seven different organs were collected during post mortem for the detection of C. pseudotuberculosis The result indicated 3 positive samples in lymph nodes, 5 in the brain and 1 in the liver. The PCR used in the present study may successfully be applied for the detection and diagnosis of C. pseudotuberculosis in the brain of the mice following oral inoculation.


Download File

[img]
Preview
PDF (Abstract)
Polymerase chain reaction detection of C.pdf

Download (84kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Institute of Bioscience
Publisher: Maxwell Science Organization
Keywords: Brain; C. pseudotuberculosis; Detection; Mice; Oral inoculation; PCR
Depositing User: Nabilah Mustapa
Date Deposited: 04 Jul 2015 08:27
Last Modified: 11 Feb 2016 14:10
URI: http://psasir.upm.edu.my/id/eprint/29843
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item