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Genotypically different clones of Staphylococcus aureus are diverse in the antimicrobial susceptibility patterns and biofilm formations

Atshan, Salman Sahab and Shamsudin, Mariana Nor and Than, Leslie Thian Lung and Sekawi, Zamberi and Chong, Pei Pei and Karunanidhi, Arunkumar and Jeevajothi Nathan, Jayakayatri and Mateg Ali, Alreshidi and Ghaznavi-Rad, Ehsanollah and Abduljaleel, Salwa and Awang Hamat, Rukman (2013) Genotypically different clones of Staphylococcus aureus are diverse in the antimicrobial susceptibility patterns and biofilm formations. BioMed Research International, 2013 . art. no. 515712. pp. 1-10. ISSN 2314-6133; ESSN: 2314-6141

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Official URL: http://dx.doi.org/10.1155/2013/515712

Abstract

This study evaluated whether genotypically different clinical isolates of S. aureus have similar susceptibilities to individual antibiotics. It further aims to check the impact of biofilm on the in vitro activity of vancomycin, daptomycin, linezolid, and tigecycline against S. aureus clones. The study used a total of 60 different clinical MSSA and MRSA isolates. Susceptibilities were performed in planktonic cultures by macrobroth dilution and epsilon-test (E test) system. Biofilm production was determined using an adherent plate assay. The efficacy of antimicrobial activities against biofilms formation was checked using confocal laser scanning microscopy (CLSM). The study found that similar and different spa, MLST, and SCCmec types displayed high variation in their susceptibilities to antibiotics with tigecycline and daptomycin being the most effective. The biofilms were found resistant to high concentrations of most antibiotics tested with daptomycin being the most effective drug used in adhesive biofilms. A considerable difference exists among similar and various clone types against antibiotics tested. This variation could have contributed to the degree of virulence even within the same clonal genotype and enhanced heterogeneity in the infection potential. Thus, the development of a rapid and precise identification profile for each clone in human infections is important.

Item Type:Article
Keyword:Staphylococcus aureus; Biofilm; Genotypically; Antimicrobial susceptibility
Faculty or Institute:Faculty of Medicine and Health Science
Institute of Bioscience
Publisher:Hindawi Publishing Corporation
DOI Number:10.1155/2013/515712
ID Code:29793
Deposited By: Raja Norazlinda Raja Azenam
Deposited On:27 Dec 2014 20:17
Last Modified:12 Feb 2016 12:22

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