Citation
Kho, Siew Leng and Chua, Kek Heng and George, Elizabeth and Tan, Mary Anne Jin Ai
(2013)
High throughput molecular confirmation of β-thalassemia mutations using novel TaqMan probes.
Sensors, 13 (2).
pp. 2506-2514.
ISSN 1424-8220
Abstract
β-Thalassemia is a public health problem where 4.5% of Malaysians are β-thalassemia carriers. The genetic disorder is caused by defects in the β-globin gene complex which lead to reduced or complete absence of β-globin chain synthesis. Five TaqMan genotyping assays were designed and developed to detect the common β-thalassemia mutations in Malaysian Malays. The assays were evaluated with 219 “blinded” DNA samples and the results showed 100% sensitivity and specificity. The in-house designed TaqMan genotyping assays were found to be cost- and time-effective for characterization of β-thalassemia mutations in the Malaysian population.
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Official URL or Download Paper: http://www.mdpi.com/1424-8220/13/2/2506
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Medicine and Health Science |
| DOI Number: | https://doi.org/10.3390/s130202506 |
| Publisher: | MDPI |
| Keywords: | β-thalassemia; Malaysia; Malay; Quantitative real-time PCR; TaqMan |
| Depositing User: | Nurul Ainie Mokhtar |
| Date Deposited: | 24 May 2015 23:50 |
| Last Modified: | 30 Nov 2016 02:47 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/s130202506 |
| URI: | http://psasir.upm.edu.my/id/eprint/29672 |
| Statistic Details: | View Download Statistic |
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