Keyword Search:


Bookmark and Share

In vitro tuberization of Chlorophytum borivilianum Sant & Fern (Safed musli) as influenced by sucrose, CCC and culture systems

Ashraf, Mehdi Farshad and Abdul Aziz, Maheran and Abdul Kadir, Mihdzar and Stanslas, Johnson and Farokhian, Elmira (2013) In vitro tuberization of Chlorophytum borivilianum Sant & Fern (Safed musli) as influenced by sucrose, CCC and culture systems. Plant & Cell Physiology, 54 (8). pp. 1356-1364. ISSN 0032-0781; ESSN: 1471-9053

[img] PDF (Abstract)
49Kb

Official URL: http://pcp.oxfordjournals.org/content/54/8/1356.ab...

Abstract

This study focuses on the establishment of in vitro tuberization of Chlorophytum borivilianum using solid and liquid culture systems. A high in vitro tuberization rate on solid and stationary liquid Murashige and Skoog media was observed in the presence of 60 g l−1 sucrose with 950, 1,265 and 1,580 µM 2-chloroethyl-trimethylammonium chloride (CCC). Application of a higher sucrose concentration of 90 g l−1 showed a negative interaction with CCC on in vitro tuber number and days to in vitro tuber induction. For economic feasibility, 950 µM CCC with 60 g l−1 sucrose was chosen as the best combination for in vitro tuberization in both solid and stationary liquid media. For optimization of in vitro tuber production, a comparison between solid, stationary liquid and shake liquid culture was carried out. Liquid culture with shaking at 80 r.p.m. resulted in a >2.5-fold increase in in vitro tuber production compared with solid culture.

Item Type:Article
Keyword:Chlorophytum borivilianum; Hyperhydricity; In vitro tuberization; Liquid culture; Solid culture
Faculty or Institute:Faculty of Agriculture
Faculty of Environmental Studies
Faculty of Medicine and Health Science
Institute of Tropical Agriculture
Publisher:Oxford University Press
DOI Number:10.1093/pcp/pct083
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1093/pcp/pct083
ID Code:29457
Deposited By: Nurul Ainie Mokhtar
Deposited On:12 Jul 2016 17:35
Last Modified:12 Jul 2016 17:35

Repository Staff Only: Edit item detail