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Recovery of lipase derived from Burkholderia cenocepacia ST8 using sustainable aqueous two-phase flotation composed of recycling hydrophilic organic solvent and inorganic salt

Anuar, Mohd Shamsul and Ariff, Arbakariya and Yusof, Yus Aniza and Chen, Soo Kien and Pau, Loke Show and Mohamad Annuar, Mohamad Suffian and Tau, Chuan Ling (2013) Recovery of lipase derived from Burkholderia cenocepacia ST8 using sustainable aqueous two-phase flotation composed of recycling hydrophilic organic solvent and inorganic salt. Separation and Purification Technology, 110 . pp. 112-118. ISSN 1383-5866

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Official URL: http://dx.doi.org/10.1016/j.seppur.2013.03.018

Abstract

Recycling hydrophilic organic solvent/inorganic salt aqueous two-phase flotation (ATPF) is a novel, low cost, green and high efficient technique for recovery of biomolecules. Recycling ATPF composed of 2-propanol and potassium phosphate was developed for sustainable separation, concentration and purification of Burkholderia cenocepacia ST8 lipase from liquid fermentation broth. Thirteen parameters upon recycling hydrophilic organic solvent/inorganic salt ATPF performance were investigated. The optimum conditions for this recycling ATPF were determined to be 40 mL volume of 50% (w/w) 2-propanol, 1.0 L of 250 g/L of potassium phosphate, pH 8.5, 100% (v/v) of crude feedstock, 30 mL/min of N2 flow rate for 30 min in a 8 cm radius of colorimeter tube with G4 porosity (5–15 μm) sintered glass disk. A purification factor of 14.4 ± 0.04 and a lipase yield of 99.2 ± 0.03% were achieved in this optimized ATPF. The recycling of phase-forming components employed at the end of recovery process was based on the principals of green chemistry, with high efficiency and economical viability. There was no gross variation of results during the process of scaling-up. Therefore, this novel recycling ATPF is feasible to be applied at industrial-scale.

Item Type:Article
Keyword:Enzymes; Biocatalysis; Alcohols; Aalt effect
Faculty or Institute:Faculty of Engineering
Publisher:Elsevier
DOI Number:10.1016/j.seppur.2013.03.018
ID Code:28475
Deposited By: Muizzudin Kaspol
Deposited On:01 Jul 2014 15:09
Last Modified:11 Feb 2016 14:41

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