Recovery of lipase derived from Burkholderia cenocepacia ST8 using sustainable aqueous two-phase flotation composed of recycling hydrophilic organic solvent and inorganic salt
Anuar, Mohd Shamsul and Ariff, Arbakariya and Yusof, Yus Aniza and Chen, Soo Kien and Pau, Loke Show and Mohamad Annuar, Mohamad Suffian and Tau, Chuan Ling (2013) Recovery of lipase derived from Burkholderia cenocepacia ST8 using sustainable aqueous two-phase flotation composed of recycling hydrophilic organic solvent and inorganic salt. Separation and Purification Technology, 110 . pp. 112-118. ISSN 1383-5866
Official URL: http://dx.doi.org/10.1016/j.seppur.2013.03.018
Recycling hydrophilic organic solvent/inorganic salt aqueous two-phase flotation (ATPF) is a novel, low cost, green and high efficient technique for recovery of biomolecules. Recycling ATPF composed of 2-propanol and potassium phosphate was developed for sustainable separation, concentration and purification of Burkholderia cenocepacia ST8 lipase from liquid fermentation broth. Thirteen parameters upon recycling hydrophilic organic solvent/inorganic salt ATPF performance were investigated. The optimum conditions for this recycling ATPF were determined to be 40 mL volume of 50% (w/w) 2-propanol, 1.0 L of 250 g/L of potassium phosphate, pH 8.5, 100% (v/v) of crude feedstock, 30 mL/min of N2 flow rate for 30 min in a 8 cm radius of colorimeter tube with G4 porosity (5–15 μm) sintered glass disk. A purification factor of 14.4 ± 0.04 and a lipase yield of 99.2 ± 0.03% were achieved in this optimized ATPF. The recycling of phase-forming components employed at the end of recovery process was based on the principals of green chemistry, with high efficiency and economical viability. There was no gross variation of results during the process of scaling-up. Therefore, this novel recycling ATPF is feasible to be applied at industrial-scale.
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