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Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source


Citation

Zakaria @ Mamat, Mohd Rafein and Ariffin, Hidayah and Abd. Aziz, Suraini and Hassan, Mohd Ali and Shirai, Yoshihito (2013) Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source. BioMed Research International, 2013. art. no. 237806. pp. 1-7. ISSN 2314-6133; ESSN: 2314-6141

Abstract

This study presents the effect of carbon to nitrogen ratio (C/N) (mol/mol) on the cell growth and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation by Comamonas sp. EB172 in 2 L fermenters using volatile fatty acids (VFA) as the carbon source. This VFA was supplemented with ammonium sulphate and yeast extract in the feeding solution to achieve C/N (mol/mol) 5, 15, 25, and 34.4, respectively. By extrapolating the C/N and the source of nitrogen, the properties of the polymers can be regulated. The number average molecular weight (M n) of P(3HB-co-3HV) copolymer reached the highest at 838 × 10 3 Da with polydispersity index (PDI) value of 1.8, when the culture broth was supplemented with yeast extract (C/N 34.4). Tensile strength and Young's modulus of the copolymer containing 6-8 mol% 3HV were in the ranges of 13-14.4 MPa and 0.26-0.34 GPa, respectively, comparable to those of polyethylene (PE). Thus, Comamonas sp. EB172 has shown promising bacterial isolates producing polyhydroxyalkanoates from renewable carbon materials.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Engineering
DOI Number: https://doi.org/10.1155/2013/237806
Publisher: Hindawi Publishing Corporation
Keywords: Poly(3-hydroxybutyrate-co-3-hydroxyvalerate); Comamonas sp. EB172; Volatile fatty acids; Carbon to nitrogen ratio
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 20 Jun 2016 03:06
Last Modified: 20 Jun 2016 03:06
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2013/237806
URI: http://psasir.upm.edu.my/id/eprint/28068
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