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Comparison of gene nature used in real-time PCR for porcine identification and quantification: a review


Citation

Mohamad, Nurhidayatul Asma and El Sheikha, Aly Farag and Mustafa, Shuhaimi and Khairil Mokhtar, Nur Fadhilah (2013) Comparison of gene nature used in real-time PCR for porcine identification and quantification: a review. Food Research International, 50 (1). pp. 330-338. ISSN 0963-9969; ESSN: 1873-7145

Abstract

Pork adulteration has been a major concern nowadays for Halal verification. Unintentional pork inclusion by contamination in highly processed food materials involves a minute amount of porcine DNA to be detected, emphasizing the need of specific and sensitive method for porcine detection. Real-time PCR is a widely used technique for species identification that can serve this purpose besides providing a powerful quantification method. Incorporation of a highly sensitive and specific probe can greatly improve the specificity and sensitivity of the assay. However, derivation of PCR primers, either from nuclear DNA (nDNA) or mitochondrial DNA (mtDNA) can relatively affect the sensitivity and specificity of the reaction as well as the quantitative measurement. In this review, both types of DNA are compared in terms of their characteristics and their influence on species identification and quantification using real-time PCR.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Halal Products Research Institute
DOI Number: https://doi.org/10.1016/j.foodres.2012.10.047
Publisher: Elsevier
Keywords: Gene nature; Real-time PCR; Halal verification; nDNA; mtDNA
Depositing User: Nabilah Mustapa
Date Deposited: 21 Apr 2016 04:28
Last Modified: 21 Apr 2016 04:28
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.foodres.2012.10.047
URI: http://psasir.upm.edu.my/id/eprint/28064
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