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Tests on the centrifugal flotation technique and its use in estimating the prevalence of Toxocara in soil samples from urban and suburban areas of Malaysia


Citation

Loh, A. G. and Israf Ali, Daud Ahmad (1998) Tests on the centrifugal flotation technique and its use in estimating the prevalence of Toxocara in soil samples from urban and suburban areas of Malaysia. Journal of Helminthology, 72 (1). pp. 39-42. ISSN 0022-149X; ESSN: 1475-2697

Abstract

The influence of soil texture (silt, sand and laterite) and flotation solutions (saturated NaCl, sucrose, NaNO3 and ZnSO4) upon the recovery of Toxocara ova from seeded soil samples with the centrifugal flotation technique was investigated. Soil samples of different texture were artificially seeded with Toxocara spp. ova and subjected to a centrifugal flotation technique which used various flotation solutions. The results showed significant (P<0.001) interactions between the soil types and the flotation solutions. The highest percentage of ova recovery was obtained with silty soil (34.9−100.8%) with saturated NaC1 as the flotation solution (45.3−100.8%). A combination of washing of soil samples with 0.1% Tween 80, and flotation using saturated NaCl and a 30 min coverslip recovery period was used to study the prevalence of contamination of soil samples. Forty-six soil samples were collected from up to 24 public parks/playgrounds in urban areas of Petaling Jaya and suburban areas of Serdang. The prevalence of Toxocara species in the urban and suburban areas was 54.5% and 45.8% respectively.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.1017/S0022149X0000095X
Publisher: Cambridge University Press
Keywords: Soil texture; Floatation solutions; Toxocara; Soil samples
Depositing User: Azizan Arshad
Date Deposited: 10 Oct 2013 06:54
Last Modified: 24 Oct 2019 08:12
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1017/S0022149X0000095X
URI: http://psasir.upm.edu.my/id/eprint/25577
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