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In situ hybridization and polymerase chain reaction methods for the detection of Epstein-Barr virus RNA in breast cancer specimens


Citation

Arif Shah, Izham Naquiddin Shah and Hassan, Zuridah and Sukor, Rashidah and Othman, Azizon and Allaudin, Zeenathul Nazariah (2011) In situ hybridization and polymerase chain reaction methods for the detection of Epstein-Barr virus RNA in breast cancer specimens. African Journal of Microbiology Research, 5 (25). art. no. 21087DE15252. pp. 4392-4397. ISSN 1996-0808

Abstract / Synopsis

The role of Epstein-Barr virus (EBV) as a cofactor in breast cancer is controversial and its association with breast cancer varies. In this study, EBV was detected by using in situhybridization technique (ISH) to detect Epstein-barr virus encoded RNA1 (EBER1) transcripts. Archival formalin-fixed paraffin embedded breast cancer tissue samples (n = 139) and normal breast tissue (n = 20) obtained from Hospital Tuanku Ja’afar were sectioned, stained and examined microscopically for nuclear staining and by DNA amplification of the same gene. By ISH, 83/139 (59.7%) and 12/20 (60%) were EBV positives in the breast cancer tissues and normal tissues, respectively. On the other hand, confirmation by polymerase chain reaction (PCR) found that additional six samples (89/139 or 64%) breast cancer tissues were positive for EBER1 gene. To further confirm the identity of these amplified products, two samples (UiTM-53 and UiTM-73) were sequenced, BLAST, analyzed phylogenetically and was found to be 100% similar to the EBV EBER1 gene sequences already deposited in the GenBank (accession numbers AB065135, FN545286, EF187853 and DQ883818). These preliminary findings suggest that there is a correlation between EBV and breast cancer but need further testing with more samples to confirm the role of EBV.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Publisher: Academic Journals
Keywords: Breast cancer; Epstein-Barr virus; EBER1 transcripts; In situ hybridization; Polymerase chainreaction (PCR); Tumour grading
Depositing User: Nur Farahin Ramli
Date Deposited: 22 Nov 2013 15:20
Last Modified: 16 Nov 2017 17:23
URI: http://psasir.upm.edu.my/id/eprint/25408
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