In situ hybridization and polymerase chain reaction methods for the detection of Epstein-Barr virus RNA in breast cancer specimens
Arif Shah, Izham Naquiddin Shah and Hassan, Zuridah and Sukor, Rashidah and O., Azizon and Alauddin, Zeenathul Nazariah (2011) In situ hybridization and polymerase chain reaction methods for the detection of Epstein-Barr virus RNA in breast cancer specimens. African Journal of Microbiology Research, 5 (25). pp. 4392-4397. ISSN 1996-0808
Official URL: http://www.academicjournals.org/journal/AJMR/artic...
The role of Epstein-Barr virus (EBV) as a cofactor in breast cancer is controversial and its associationwith breast cancer varies. In this study, EBV was detected by using in situ hybridization technique (ISH)to detect Epstein-barr virus encoded RNA1 (EBER1) transcripts. Archival formalin-fixed paraffinembedded breast cancer tissue samples (n = 139) and normal breast tissue (n = 20) obtained from Hospital Tuanku Ja’afar were sectioned, stained and examined microscopically for nuclear staining andby DNA amplification of the same gene. By ISH, 83/139 (59.7%) and 12/20 (60%) were EBV positives inthe breast cancer tissues and normal tissues, respectively. On the other hand, confirmation bypolymerase chain reaction (PCR) found that additional six samples (89/139 or 64%) breast cancertissues were positive for EBER1 gene. To further confirm the identity of these amplified products, twosamples (UiTM-53 and UiTM-73) were sequenced, BLAST, analyzed phylogenetically and was found tobe 100% similar to the EBV EBER1 gene sequences already deposited in the GenBank (accessionnumbers AB065135, FN545286, EF187853 and DQ883818). These preliminary findings suggest that thereis a correlation between EBV and breast cancer but need further testing with more samples to confirmthe role of EBV.
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