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Identification of adipogenesis and osteogenesis pathways of differentiated bone marrow stem cells in vitro in rabbits

Al-Timmemi, Hameed A. and Ibrahim, Rashid and Al-Jashamy, Karim Alwan Mohamed and Abu Bakar @ Zakaria, Md Zuki and Tengku Ibrahim, Tengku Azmi and Ramasamy, Rajesh (2011) Identification of adipogenesis and osteogenesis pathways of differentiated bone marrow stem cells in vitro in rabbits. Annals of Microscopy, 11 . pp. 24-29. ISSN 0219-2209

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Abstract

Transdifferentiation is a process whereby a cell type committed to and progressing along a specific developmental lineage switches into another cell types. The objective of this study was to assess whether rabbit mesenchymal stem cells (rMSCs) precommitted to give mesenchymal cell lineage transdifferentiate in response to inductive extracellular cues to expand adult MSCs. Bone mesenchymal stem cells (BMSCs) obtained from ilium of adult male rabbit comprised heterogeneous groups of cells after seeding and growing in culture plates. After initial plating, the adherent cells exhibited small rounded, spindle-shaped and exhibited fibroblast-like morphology in reaching confluence. Rabbit BMSCs differentiated into adipocytes and osteocyte as a accumulation of intracellular lipid droplets and calcium deposition throughout the culture after 21 days.

Item Type:Article
Keyword:Adipogenesis; Osteogenesis; MSC
Faculty or Institute:Faculty of Veterinary Medicine
Publisher:Microscopy Society (Singapore)
ID Code:25392
Deposited By: Nur Farahin Ramli
Deposited On:22 Nov 2013 17:01
Last Modified:18 Nov 2016 09:23

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