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Effects of CXCR4 siRNA/dextran-spermine nanoparticles on CXCR4 expression and serum LDH levels in a mouse model of colorectal cancer metastasis to the liver.

Abedini, Fatemeh and Ismail, Maznah and Hosseinkhani, Hossein and Tengku Ibrahim, Tengku Azmi and Omar, Abdul Rahman and Chong, Pei Pei and Bejo, Mohd Hair and Domb, Abraham J. (2011) Effects of CXCR4 siRNA/dextran-spermine nanoparticles on CXCR4 expression and serum LDH levels in a mouse model of colorectal cancer metastasis to the liver. Cancer Management and Research, 3 (-). pp. 301-309. ISSN 1179-1322

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Abstract

Liver metastasis is the main cause of mortality related to colorectal cancer. CXCR4 is necessary for the outgrowth of colon cancer micrometastases. In oncology, it has been demonstrated that several human tumors release lactate dehydrogenase (LDH) into the circulation. CXCR4 gene expression and serum LDH levels are often increased in patients with colorectal cancer. Despite technological advances in cancer therapy, five-year overall survival is still around 50%. Therefore, better treatment needs to be developed. RNA interference (RNAi) is a modern and powerful tool for inhibition of gene expression. However, the rate-limiting step in this technology is effective delivery of RNAi agents. We have investigated a novel strategy of CXCR4 siRNA therapy and its effect on serum LDH levels in a BALB/C mouse model of colorectal cancer metastasis to the liver. Hepatic metastasis was established by injecting a CT26.WT mouse colon carcinoma cell line via the tail vein. Our results demonstrated that CXCR4 siRNA/ dextran-spermine nanoparticles achieved high silencing efficiency with low toxicity. Favorable localization of the nanoparticles was confirmed with CXCR4 gene expression in the liver, that was correlated with serum LDH levels. More research will be needed to determine the effect of CXCR4 silencing on serum LDH levels, which may be a useful marker for predicting liver metastasis in colorectal cancer.

Item Type:Article
Keyword:Colorectal cancer; CXCR4 siRNA; Serum LDH; Liver; Dextran-spermine.
Faculty or Institute:Faculty of Veterinary Medicine
DOI Number:10.2147/CMR.S11678
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.2147/CMR.S11678
ID Code:25377
Deposited By: Nur Farahin Ramli
Deposited On:10 Jul 2013 15:58
Last Modified:29 Sep 2015 15:32

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