Keyword Search:


Bookmark and Share

Effect of butylated hydroxytoluene on cryopreservation of Boer goat semen in Tris egg yolk extender.

Ahmed Memon, Akeel and H., Wahid and Yusoff, Rosnina and Goh, Yong Meng and Ebrahimi, Mahdi and Nadia, F.M. and Audrey, G. (2011) Effect of butylated hydroxytoluene on cryopreservation of Boer goat semen in Tris egg yolk extender. Animal Reproduction Science, 129 (1-2). pp. 44-49. ISSN 0378-4320

[img] PDF (Abstract)
83Kb

Official URL: http://www.elsevier.com/

Abstract

The aim of this study was to determine the effect of butylated hydroxytoluene (BHT), a lipid-soluble anti-oxidant added in different concentrations to the Tris egg yolk extenders on semen cytological parameters pre freezing and post thawing (motility, morphology, viability, acrosome integrity and membrane integrity) of Boer goat spermatozoa. A total of 40 ejaculates from four Boer goat bucks were collected using an artificial vagina. Ten replicates of the ejaculates were diluted with a Tris egg yolk based extender which contained various concentrations (0.5mM, 1.0mM, 2.0mM and 3.0mM) of butylated hydroxytoluene while one sample was processed without supplementation of antioxidant and served as control. The diluted semen was cooled at 4°C and loaded into the straw and then stored in liquid nitrogen. It was evident that supplementation of BHT produces positive effect in terms of motility, membrane integrity and acrosome integrity in comparison with the control group in cooled and frozen Boer goat semen. Results showed significant differences in motility, membrane integrity, acrosome integrity and viability of cooled and frozen Boer goat spermatozoa at different concentrations. Motility, membrane integrity, acrosome integrity and viability was significantly higher in all treated groups than the control group (P<0.05) while there was no significant differences (P>0.05) in morphology trait between all group in cooled semen. However, improvement (P<0.05) was observed only in terms of the membrane integrity and acrosome integrity compared to the control and other treated groups in frozen semen. In conclusion, BHT can be used in cryopreservation of Boer goat semen in order to reduce the oxidative stress on spermatozoa.

Item Type:Article
Keyword:Butylated hydroxytoluene; Goat; Semen; Freezing; Extenders; Antioxidants.
Faculty or Institute:Faculty of Veterinary Medicine
Publisher:Elsevier
DOI Number:10.1016/j.anireprosci.2011.10.004
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.anireprosci.2011.10.004
ID Code:25374
Deposited By: Nur Farahin Ramli
Deposited On:12 Jul 2013 09:59
Last Modified:08 Oct 2015 12:38

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 12 Jul 2013 09:59.

View statistics for "Effect of butylated hydroxytoluene on cryopreservation of Boer goat semen in Tris egg yolk extender."