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Assessment of sensitivity, specificity and species discriminatory power of four culture-based isolation methods of Arcobacter spp


Citation

Hussain Shah, Sayed Atta and Abdul Aziz, Saleha and Marimuthu, Murugaiyah and Zakaria, Zunita (2011) Assessment of sensitivity, specificity and species discriminatory power of four culture-based isolation methods of Arcobacter spp. African Journal of Microbiology Research, 5 (22). art. no. 08A5A0513572. pp. 3753-3759. ISSN 1996-0808

Abstract

A total of 142 samples including beef (n = 52), fresh milk (n = 45) and rectal swabs of cattle (n = 45) were collected from various wet markets (beef) and dairy cattle farms (milk and rectal swabs) in the vicinity of Selangor, Malaysia. All samples were examined for the presence of Arcobacter species using four different isolation methods. The organisms were first identified on the basis of phenotypic tests and later the suspected isolates were confirmed using multiplex PCR (mPCR). Method I (MI) and Method II (MII) detected Arcobacter in 43.7 and 75%, respectively from beef, but were unable to detect microbe from milk and rectal swabs. On the other hand, Method III (MIII) detected Arcobacter in 100, 60 and 40% of beef, milk and bovine rectal swab samples respectively; Method IV (MIV) detected 93.7, 60 and 40% in beef, milk and cattle rectal swabs respectively. The discriminatory power among the isolation methods for Arcobacter species was evaluated and MIII was found to be the best as it identified A. butzleri (72.7%); A. cryaerophilus (22.7%) and A. skirrowii (4.5%) while A. butzleri (61.1%), A. cryaerophilus (22.7%) and A. skirrowii (9%) were isolated from MIV.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.5897/AJMR11.618
Publisher: Academic Journals
Keywords: Arcobacter; Isolation methods; mPCR; Beef; Milk; Cattle
Depositing User: Nur Farahin Ramli
Date Deposited: 23 Oct 2013 03:01
Last Modified: 22 Nov 2016 09:07
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5897/AJMR11.618
URI: http://psasir.upm.edu.my/id/eprint/25319
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