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Effect of alcohol structure on the optimum condition for Novozym 435-catalyzed synthesis of adipate esters.

Abdul Rahman, Mohd Basyaruddin and Chaibakhsh, Naz and Basri, Mahiran (2011) Effect of alcohol structure on the optimum condition for Novozym 435-catalyzed synthesis of adipate esters. Biotechnology Research International, 2011 (162987). pp. 1-7. ISSN 2090-3138; ESSN:2090-3146

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Abstract

Immobilized Candida antarctica lipase B, Novozym 435, was used as the biocatalyst in the esterification of adipic acid with four different isomers of butanol (n-butanol, sec-butanol, iso-butanol, and tert-butanol). Optimum conditions for the synthesis of adipate esters were obtained using response surface methodology approach with a four-factor-five-level central composite design concerning important reaction parameters which include time, temperature, substrate molar ratio, and amount of enzyme. Reactions under optimized conditions has yielded a high percentage of esterification (>96%) for n-butanol, iso-butanol, and sec-butanol, indicating that extent of esterification is independent of the alcohol structure for primary and secondary alcohols at the optimum conditions. Minimum reaction time (135 min) for achieving maximum ester yield was obtained for iso-butanol. The required time for attaining maximum yield and also the initial rates in the synthesis of di-n-butyl and di-sec-butyl adipate were nearly the same. Immobilized Candida antarctica lipase B was also capable of esterifying tert-butanol with a maximum yield of 39.1%. The enzyme is highly efficient biocatalyst for the synthesis of adipate esters by offering a simple production process and a high esterification yield.

Item Type:Article
Keyword:Candida antarctica lipase B; Adipate esters; Esterification; Response surface methodology.
Faculty or Institute:Faculty of Science
Publisher:Hindawi Publishing Corporation
DOI Number:10.4061/2011/162987
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.4061/2011/162987
ID Code:25184
Deposited By: Nur Farahin Ramli
Deposited On:16 Jul 2013 14:59
Last Modified:08 Oct 2015 12:41

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