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Efficient in vitro regeneration of Zingiber zerumbet Smith (a valuable medicinal plant) plantlets from rhizome bud explants


Citation

Qamaruz Zaman, Faridah and Abdelmageed, Adil Hassan Ahmed and Abd Aziz, Julia and R., Nor Hafizah (2011) Efficient in vitro regeneration of Zingiber zerumbet Smith (a valuable medicinal plant) plantlets from rhizome bud explants. African Journal of Biotechnology, 10 (46). art. no. B01A6C226273. pp. 9303-9308. ISSN 1684–5315

Abstract / Synopsis

This study was conducted to develop an efficient protocol for mass propagation ofZingiber zerumbet Smith. Explants from rhizome buds were cultured on Murashige and Skoog (MS) medium supplemented with 6-Benzylaminopurine (BAP) alone (0 to 5 mg/l) or a combination of BAP (0 to 5 mg/l) and indole 3-acetic acid (IAA) (0 to 2 mg/l). MS medium supplemented with a combination of 5.0 mg/l BAP and 2.0 mg/l IAA or 3.0 mg/l BAP and 0.5 mg/l IAA produced the highest mean number of shoots (5.6) per explant as compared to other concentrations. The best shoots length (9.44 cm) was obtained on the medium containing 1.0 mg/l of BAP and 2.0 mg/l IAA. Thus, combined effects of BAP and IAA improved significantly the shoot growth and proliferation. MS medium supplemented with a combination of 5.0 mg/l BAP and 2 mg/l IAA gave the highest number of roots (17). However, longest roots per explant were obtained with 1.0 mg/l BAP alone. The proliferated shoots were green and healthy in appearance. Finally, healthy and complete plants with well developed roots were hardened, acclimatized and planted in the field successfully with a survival rate of 80%.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
Institute of Bioscience
DOI Number: 10.5897/AJB11.1182
Publisher: Academic Journals
Keywords: Zingiber zerumbet; Shoot multiplication; Micropropagation; In vitro; Acclimatization; Medicinal plants; Zingiberaceae
Depositing User: Nur Farahin Ramli
Date Deposited: 02 Oct 2013 14:51
Last Modified: 30 Jun 2016 10:57
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5897/AJB11.1182
URI: http://psasir.upm.edu.my/id/eprint/24252
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