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Efficient in vitro regeneration of Zingiber zerumbet Smith (a valuable medicinal plant) plantlets from rhizome bud explants

Qamaruz Zaman, Faridah and Abdelmageed, Adil Hassan Ahmed and Abd Aziz, Julia and R., Nor Hafizah (2011) Efficient in vitro regeneration of Zingiber zerumbet Smith (a valuable medicinal plant) plantlets from rhizome bud explants. African Journal of Biotechnology, 10 (46). art. no. B01A6C226273. pp. 9303-9308. ISSN 1684–5315

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Abstract

This study was conducted to develop an efficient protocol for mass propagation ofZingiber zerumbet Smith. Explants from rhizome buds were cultured on Murashige and Skoog (MS) medium supplemented with 6-Benzylaminopurine (BAP) alone (0 to 5 mg/l) or a combination of BAP (0 to 5 mg/l) and indole 3-acetic acid (IAA) (0 to 2 mg/l). MS medium supplemented with a combination of 5.0 mg/l BAP and 2.0 mg/l IAA or 3.0 mg/l BAP and 0.5 mg/l IAA produced the highest mean number of shoots (5.6) per explant as compared to other concentrations. The best shoots length (9.44 cm) was obtained on the medium containing 1.0 mg/l of BAP and 2.0 mg/l IAA. Thus, combined effects of BAP and IAA improved significantly the shoot growth and proliferation. MS medium supplemented with a combination of 5.0 mg/l BAP and 2 mg/l IAA gave the highest number of roots (17). However, longest roots per explant were obtained with 1.0 mg/l BAP alone. The proliferated shoots were green and healthy in appearance. Finally, healthy and complete plants with well developed roots were hardened, acclimatized and planted in the field successfully with a survival rate of 80%.

Item Type:Article
Keyword:Zingiber zerumbet; Shoot multiplication; Micropropagation; In vitro; Acclimatization; Medicinal plants; Zingiberaceae
Faculty or Institute:Faculty of Science
Institute of Bioscience
Publisher:Academic Journals
DOI Number:10.5897/AJB11.1182
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5897/AJB11.1182
ID Code:24252
Deposited By: Nur Farahin Ramli
Deposited On:02 Oct 2013 14:51
Last Modified:30 Jun 2016 10:57

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