UPM Institutional Repository

Immunomodulatory effect of Rhaphidophora korthalsii on natural killer cell cytotoxicity.


Citation

Yeap, Swee Keong and Omar, Abdul Rahman and Ali, Abdul Manaf and Ho, Wan Yong and Beh, Boon Kee and Mohamed Alitheen, Noorjahan Banu (2012) Immunomodulatory effect of Rhaphidophora korthalsii on natural killer cell cytotoxicity. Evidence-Based Complementary and Alternative Medicine, 2012 (786487). pp. 1-7. ISSN 1741-427X; ESSN: 1741-4288

Abstract

The in vivo immunomodulatory effect of ethanolic extracts from leaves of Rhaphidophora korthalsii was determined via immune cell proliferation, T/NK cell phenotyping, and splenocyte cytotoxicity of BALB/c mice after 5 consecutive days of i.p. administration at various concentrations. Splenocyte proliferation index, cytotoxicity, peripheral blood T/NK cell population, and plasma cytokine (IL-2 and IFN-γ) in mice were assessed on day 5 and day 15. High concentration of extract (350μg/mice/day for 5 consecutive days) was able to stimulate immune cell proliferation, peripheral blood NK cell population, IL-2, and IFN-γ cytokines, as well as splenocyte cytotoxicity against Yac-1 cell line. Unlike rIL-2 which degraded rapidly, the stimulatory effect from the extract managed to last until day 15. These results suggested the potential of this extract as an alternative immunostimulator, and they encourage further study on guided fractionation and purification to identify the active ingredients that contribute to this in vitro and in vivo immunomodulatory activity.


Download File

[img]
Preview
PDF (Abstract)
Immunomodulatory effect of Rhaphidophora korthalsii on natural killer cell cytotoxicity.pdf

Download (149kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Veterinary Medicine
Institute of Bioscience
DOI Number: https://doi.org/10.1155/2012/78648
Publisher: Hindawi Publishing Corporation
Keywords: Immunomodulatory; Natural killer cells; Rhaphidophora korthalsii.
Depositing User: Nur Farahin Ramli
Date Deposited: 25 Feb 2014 00:15
Last Modified: 27 Oct 2015 03:24
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2012/78648
URI: http://psasir.upm.edu.my/id/eprint/24114
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item