Citation
Abstract
Malaysia, Biosafety Bill 2006 was approved by Parliament in July 2007, and labeling legislation will be implemented soon. In this study, duplex polymerase chain reaction (PCR) was carried out to detect endogenous soybean lectin gene and exogenous cp4-epsps (5’-enolpyruvylshikimate-3-phospate synthase) gene simultaneously. Additionally, real-time PCR utilizing SYBR Green fluorescence dye were established for the quantitative analysis of Roundup Ready soybean (RRS), which is based on the two established calibration curve from cloned fragment of cp4-epsps gene and lectin gene respectively. Approximately, 39.5% (45/114) of the samples examined in this study contain RRS, animal feeds (31), processed food (13) and raw soybean (1). Additionally, 75.6% (34/45) of the positive samples were found contained RRS above 0.9%. The sensitive GMO quantitative approach described in this study enable the analysis of various samples and this will facilitate the labeling process.
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Official URL or Download Paper: http://www.ifrj.upm.edu.my/volume-18-2011.html
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Food Science and Technology Faculty of Medicine and Health Science |
| DOI Number: | http://www.ifrj.upm.edu.my/18%20(02)%202011/(6)%20IFRJ-2010-108.pdf |
| Publisher: | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| Keywords: | Cloned DNA standard; Food and animal feed products; Genetically modified organisms; PCR; Quantitative real-time PCR; Roundup ready soybean |
| Depositing User: | Nur Farahin Ramli |
| Date Deposited: | 18 Feb 2014 06:27 |
| Last Modified: | 02 Jun 2015 02:44 |
| URI: | http://psasir.upm.edu.my/id/eprint/24061 |
| Statistic Details: | View Download Statistic |
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