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Genetic diversity of wild and cultured populations of Penaeus monodon using microsatellite markers.

Aziz, Dania and Siraj, Siti Shapor and Daud, Siti Khalijah and Panandam, Jothi Malar and Othman, Muhammad Fauzan (2011) Genetic diversity of wild and cultured populations of Penaeus monodon using microsatellite markers. Journal of Fisheries and Aquatic Science, 6 (6). pp. 614-623. ISSN 1816-4927

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Official URL: http://dx.doi.org/10.3923/jfas.2011.614.623

Abstract

Information on the genetic diversity of Penaeus monodon throughout its natural range in Malaysia is still Hmited even though it is a highly exploited species, thus this study was undertaken to genetically characterize the prawn populations. The P. monodon samples were randomly collected from Malaysian waters and were characterized using thirty polymorphic primer pairs which showed high level of polymorphism. The total number of alleles per locus ranged from 3 to 36 with allele size ranging from 100 to 275 base pairs. The mean observed heterozygosity (0.5166) was less than the expected (0.5552), highly significant deficiencies in heterozygotes were detected in total inbreeding (F IS = 0.5500) and pair-wise genetic differentiation (F ST = 0.6308) among the populations. Both the (χ 2) chi-square and (G 2) likelihood ratio tests detected significant differences (p<0.05) which showed a deviation from the Hardy-Weinberg equilibrium, indicating a probable inbreeding might have occurred in the populations. A Cluster analysis based on genetic distance revealed a fair genetic relationship among all the populations and the pattern was in accordance to the populations' geographical origins. The highest genetic distance (0.7588) was observed between Law as and Bulau Sayak populations while the lowest genetic distance (0.1191) was recorded between the Endau Rompin and Sedili populations. Various levels of genetic diversity of the P. monodon reported in this study indicated their genetic status in Malaysian waters and suitability for breeding and culture purposes. This information provides a basis for improvement through selective breeding and in the design of suitable management guidelines for this genetic material.

Item Type:Article
Keyword:Genetic diversity; Wild; Cultured populations; Penaeus monodon; Microsatellite markers
Faculty or Institute:Faculty of Agriculture
Publisher:Academic Journals
DOI Number:10.3923/jfas.2011.614.623
ID Code:23653
Deposited By: Nur Farahin Ramli
Deposited On:29 Nov 2013 09:02
Last Modified:10 Feb 2016 08:46

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