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Development of single-locus DNA microsatellite markers using 50anchored ISSR-PCR method for the mangrove horseshoe crab, Carcinoscorpius rotundicauda (Latreille, 1802) in Peninsular Malaysia

Abu Bakar, Adibah and Liew, Pui Ling and Tan, Soon Guan and Christianus, Annie and Qamaruz Zaman, Faridah (2012) Development of single-locus DNA microsatellite markers using 50anchored ISSR-PCR method for the mangrove horseshoe crab, Carcinoscorpius rotundicauda (Latreille, 1802) in Peninsular Malaysia. Molecular Biology Reports, 39 (3). pp. 3815-3820. ISSN 0301-4851; ESSN:1573-4978

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Official URL: http://dx.doi.org/10.1007/s11033-011-1159-6

Abstract

Horseshoe crabs are said to be declining worldwide. However, there is still no published report on the status of horseshoe crabs in Malaysia. Thus, we report here eight informative microsatellite markers that were developed using the 50-anchored ISSR-PCR enrichment procedure to diagnose the population genetic structure of the mangrove horseshoe crab, Carcinoscorpius rotundicauda from Peninsular Malaysia. This set of markers was tested on 127 samples and showed polymorphism in this species. Hence they should be useful in future essential population genetic studies of these living fossils in the Southeast Asian region.

Item Type:Article
Keyword:Mangrove horseshoe crab; C. rotundicauda; 5' anchored ISSR-PCR; Microsatellite
Faculty or Institute:Faculty of Agriculture
Publisher:Springer Link
DOI Number:10.1007/s11033-011-1159-6
ID Code:23587
Deposited By: Nur Farahin Ramli
Deposited On:10 Dec 2013 12:59
Last Modified:05 Feb 2016 12:31

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