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Phenotypic characterization and molecular identification of Malaysian Pseudomonas fuscovaginae isolated from rice plants

Ab Wahab, Mohd Zafri and Sijam, Kamaruzaman and Ismail, Mohd Razi and Hashim, Marzukhi and Mohd Hata, Erneeza and Mohamed Zulperi, Dzarifah (2015) Phenotypic characterization and molecular identification of Malaysian Pseudomonas fuscovaginae isolated from rice plants. Asian Journal of Plant Pathology, 9 (3). pp. 112-123. ISSN 1819-1541

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Abstract

Sheath Brown Rot (SBR) is one of the most important diseases that affect rice plant. The disease cause rots to the sheaths, panicles and leaves of rice plant. The disease also contaminating rice seeds through grain’s discoloration thus leads in reduction of rice production. In this study, 50 bacteria isolates that fluoresced under UV illumination on King’s B Selective (KBS) medium were obtained. Out of 50 isolates, 25 isolates were positive for both Hypersensitivity Reaction (HR) and pathogenicity tests. They also showed positive results for several biochemical reactions such as oxidase, arginine dihydrolase and trehalose utilization tests. Based on the preliminary tests, all isolates have been identified as Pseudomonas fuscovaginae (P. fuscovaginae). The 16S rDNA sequence analysis showed that all the isolates had 97-99% sequence similarities to Pseudomonas fuscovaginae in the GenBank database. This result was also supported by the constructed phylogenetic tree with the Acidivorax avenae outgroup. It was noted that all 25 isolates originated from the same node with the P. fuscovaginae group. The findings on characteristics of P. fuscovaginae in Peninsular Malaysia hopefully can be used in future studies especially on Malaysian P. fuscovaginae biology and their control.

Item Type:Article
Keyword:16S rDNA; Characterization; Malaysian isolates; Pathogenicity; Pseudomonas fuscovaginae; Sheath brown rot
Faculty or Institute:Faculty of Agriculture
Institute of Tropical Agriculture
Publisher:Academic Journals
DOI Number:10.3923/ajppaj.2015.112.123
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/ajppaj.2015.112.123
ID Code:23282
Deposited By: Nurul Ainie Mokhtar
Deposited On:10 Jun 2016 17:08
Last Modified:10 Jun 2016 17:08

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