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Bioactive compounds and antioxidant activity of different extracts from Vitex negundo leaf


Citation

Zargar, Mohsen and Abdul Hamid, Azizah and Mustafa, Roheeyati Ashikin and Abu Bakar, Fatimah and Jahanshiri, Fatemeh and Pak Dek, Mohd Sabri (2011) Bioactive compounds and antioxidant activity of different extracts from Vitex negundo leaf. Journal of Medicinal Plants Research, 5 (12). art. no. 6A593BF23500. pp. 2525-2532. ISSN 1996-0875

Abstract

The study was conducted to assess the antioxidant activity of methanol and hexane extract and essential oil from Vitex negundo leaf using different in vitro antioxidant assays. Antioxidant property was tested using 2,2-diphenyl-1-picrylhydrozyl (DPPH) free radical scavenging capacity, ferric ion reducing antioxidant power (FRAP) and β-carotene-linoleic acid assays. Total phenolic contents (TPC) were measured using Folin-Ciocalteu method. Flavonoids, tocopherols, β-carotene and lycopene were analyzed using high performance liquid chromatography (HPLC). Results of the present study showed that methanol extract of V. negundo leaf exhibited significantly (p < 0.05) higher antioxidant activity in terms of measurements of DPPH free radical (IC50), FRAP and β-carotene-linoleic assays than those of hexane extract and essential oil. Methanol extract of V. negundo leaf also contained high amounts of bioactive compounds including total phenolic compounds (363 mg GAE/g), epicatechin (16.98 mg/g), quercetin (13.45 mg/g), catechin (8.95 mg/g) and myricetin (3.32 mg/g) while the concentrations of tocopherol, β-carotene and lycopene were found to be lower.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Food Science and Technology
Publisher: Academic Journals
Keywords: Vitex negundo leaf; Antioxidant activity; Bioactive compounds; Methanol extract; Essential oil
Depositing User: Nabilah Mustapa
Date Deposited: 08 Jun 2016 01:00
Last Modified: 08 Jun 2016 01:00
URI: http://psasir.upm.edu.my/id/eprint/22434
Statistic Details: View Download Statistic

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