Citation
Abstract
In this study, we report an optimization of particle bombardment transformation system for Centella asiatica callus. A total of eight parameters affecting the genetic transformation system were optimized using the synthetic green fluorescent protein (sGFP) as a reporter driven by the CaMV 35S promoter. The results indicated that DNA delivery conditions of 9-cm target distance, 1,100 psi helium pressure, 1.0 μm gold particles size, 27 mmHg chamber vacuum pressure, 2 times number of bombardment, spermidine as precipitation agent, 60 h post-bombardment incubation time and 2 μg plasmid DNA concentration were optimal for C. asiatica callus transformation. The expression of sGFP was monitored using fluorescence microscope and further confirmed using RT-PCR. This optimized genetic transformation system is applicable for rapid transient gene analysis and transgenic C. asiatica production.
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Official URL or Download Paper: http://link.springer.com/article/10.1007%2Fs11738-...
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Institute of Bioscience |
| DOI Number: | https://doi.org/10.1007/s11738-011-0786-9 |
| Publisher: | Springer-Verlag |
| Keywords: | Centella asiatica; SGFP expression; Particle bombardment RT-PCR; Transformation |
| Depositing User: | Nurul Ainie Mokhtar |
| Date Deposited: | 07 Dec 2015 05:02 |
| Last Modified: | 07 Dec 2015 05:02 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s11738-011-0786-9 |
| URI: | http://psasir.upm.edu.my/id/eprint/22377 |
| Statistic Details: | View Download Statistic |
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