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High yield lipase-catalyzed synthesis of Engkabang fat esters for the cosmetic industry

Abd. Rahman, Nur Fariza and Basri, Mahiran and Abdul Rahman, Mohd Basyaruddin and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar (2011) High yield lipase-catalyzed synthesis of Engkabang fat esters for the cosmetic industry. Bioresource Technology, 102 (3). pp. 2168-2176. ISSN 0960-8524; ESSN: 1873-2976

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Abstract

Engkabang fat esters were produced via alcoholysis reaction between Engkabang fat and oleyl alcohol, catalyzed by Lipozyme RM IM. The reaction was carried out in a 500 ml Stirred tank reactor using heptane and hexane as solvents. Response surface methodology (RSM) based on a four-factor-five-level Central composite design (CCD) was applied to evaluate the effects of synthesis parameters, namely temperature, substrate molar ratio (oleyl alcohol: Engkabang fat), enzyme amount and impeller speed. The optimum yields of 96.2% and 91.4% were obtained for heptane and hexane at the optimum temperature of 53.9 °C, impeller speeds of 309.5 and 309.0 rpm, enzyme amounts of 4.82 and 5.65 g and substrate molar ratios of 2.94 and 3.39:1, respectively. The actual yields obtained compared well with the predicted values of 100.0% and 91.5%, respectively. Meanwhile, the properties of the esters show that they are suitable to be used as ingredient for cosmetic applications.

Item Type:Article
Keyword:Engkabang fat esters; Response surface methodology; Central composite design; Alcoholysis; Stirred tank reactor
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
Publisher:Elsevier
DOI Number:10.1016/j.biortech.2010.10.034
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.biortech.2010.10.034
ID Code:22293
Deposited By: Najwani Amir Sariffudin
Deposited On:09 Jul 2014 12:06
Last Modified:27 Sep 2016 15:03

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