Immobilised Glutamate Dehydrogenase: Possible Use In Automated Analysis.

Abstract

Glutamate dehydrogenase was immobilised onto nylon tube and the activity of the enzyme tube investigated. Two different spacer molecules and two coupling techniques were utilised for immobilising the enzyme. Relatively low activity was retained on the tube, but when the enzyme tube was incorporated into a continuous flow system, and used to assay standard ammonia solution, there was adequate response for measurement. The study indicated the potential use ofimmobilised glutamate dehydrogenase in routine analytical operation.