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The nature of apoptosis of human breast cancer cells induced by three species of genus Ganoderma P. Karst. (Aphyllophoromycetideae) crude extracts


Citation

Choong, Yew Keong and Mohamed Mustapha, Noordin and Mohamed, Suhaila and Ali, Abdul Manaf and Umar, Nor Aini and Tong, Chow Chin (2008) The nature of apoptosis of human breast cancer cells induced by three species of genus Ganoderma P. Karst. (Aphyllophoromycetideae) crude extracts. International Journal of Medicinal Mushrooms, 10 (2). pp. 115-125. ISSN 1521-9437; ESSN: 1940-4344

Abstract

The cytotoxic effect of crude extracts of three Ganoderma species (G. lucidum, G. tropicum, and G. tsugae) was screened against the human estrogen-independent cell line, MDA-MB-435 using a microculture tetrazolium (MTT) assay. The crude extract obtained from the fruiting body of Ganoderma lucidum by hot water extraction procedures had IC50 values ranging from 140 to 350 μg mL−1. Acridine-orange/propidium iodine staining, electron microscopy, and transmission electron microscopy showed that the crude extract caused both apoptosis and necrosis. The G. lucidum mycelial crude extract was most effective, with an IC50 value of 148 μg mL−1.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Food Science and Technology
Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.1615/IntJMedMushr.v10.i2.20
Publisher: Begell House
Keywords: Apoptosis; Ganoderma; IC50; Ling Zhi mushrooms; MDA-MB-435; Medicinal mushrooms; Mycelium; Necrosis
Depositing User: Nabilah Mustapa
Date Deposited: 08 Apr 2016 07:34
Last Modified: 08 Apr 2016 07:34
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1615/IntJMedMushr.v10.i2.20
URI: http://psasir.upm.edu.my/id/eprint/17248
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