Optimization of protein extraction from fish waste using Response Surface Methodology

Citation

Husin, Nurdiyana and Mustapa Kamal, Siti Mazlina (2009) Optimization of protein extraction from fish waste using Response Surface Methodology. Journal of Applied Sciences, 9 (17). pp. 3121-3125. ISSN 1812-5654; ESSN: 1812-5662

Abstract

The aim of present study was to optimize protein extraction from Fish Waste (FW), sardine (Sardina pilchardus) using enzymatic method. In this study, enzymatic method involved two combinations of enzymes which were alcalase and protamex. Response Surface Methodology (RSM) was used to study the effect of independent variables, namely temperature (35-55°C), rotation speed (100-300 rpm), time (60-1440 min) and enzyme:substrate ratio (0.5-1.5) on protein extraction from FW. From RSM-generated model, the optimum conditions for extraction of protein from FW were identified to be at temperature 35°C in 1429 min reaction time, with rotation speed of 171 rpm and enzyme:substrate ratio of 1.50. At the optimum conditions, predicted protein yield in the extraction process was 80.75 mg mL-1.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Engineering
DOI Number:	https://doi.org/10.3923/jas.2009.3121.3125
Publisher:	Asian Network for Scientific Information
Keywords:	Enzymatic method; Fish waste; Response Surface Methodology; Sardine pilchardus
Depositing User:	Fatimah Zahrah @ Aishah Amran
Date Deposited:	10 Nov 2014 02:08
Last Modified:	15 Sep 2015 07:04
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/jas.2009.3121.3125
URI:	http://psasir.upm.edu.my/id/eprint/15982
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