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Molecular investigation of a gene encoding organic solvent-tolerant alkaline protease from Pseudomonas aeruginosa strain K

Raja Abdul Rahman, Raja Noor Zaliha and Lee, Poh Geok and Wong, Chee Fah and Basri, Mahiran and Salleh, Abu Bakar (2010) Molecular investigation of a gene encoding organic solvent-tolerant alkaline protease from Pseudomonas aeruginosa strain K. Journal of Basic Microbiology, 50 (2). pp. 143-149. ISSN 0233-111X; ESSN: 1521-4028

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Official URL: http://onlinelibrary.wiley.com/wol1/doi/10.1002/jo...

Abstract

A gene encoding an organic solvent-stable protease was amplified from Pseudomonas aeruginosa strain K by polymerase chain reaction using consensus primers based on multiple sequence alignment of alkaline and metalloprotease genes from Pseudomonas species. The gene, which consisted of 1440 bp nucleotides and deduced 479 amino acid residues, was successfully expressed in pGEX-4T-1 expression system in the presence of 1.0 mM IPTG, after an incubation of 6 h at 37 degrees C. Under these conditions, the recombinant strain K protease was, subsequently, released into the periplasm of E. coli BL21 (DE3) with an optimum proteolytic activity detected at 1.0112 U/ml. To date, this is the first reported expression of alkaline protease (aprA) with such remarkable property in Escherichia coli.

Item Type:Article
Keyword:Pseudomonas aeruginosa strain K; Organic solvent-tolerant protease gene; aprA; Expression
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
Publisher:Wiley-VCH Verlag GmbH & Co. KGaA
DOI Number:10.1002/jobm.200900133
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1002/jobm.200900133
ID Code:15709
Deposited By: Nida Hidayati Ghazali
Deposited On:24 Jul 2012 11:18
Last Modified:26 Sep 2016 12:57

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