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Molecular investigation of a gene encoding organic solvent-tolerant alkaline protease from Pseudomonas aeruginosa strain K


Citation

Raja Abdul Rahman, Raja Noor Zaliha and Lee, Poh Geok and Wong, Chee Fah and Basri, Mahiran and Salleh, Abu Bakar (2010) Molecular investigation of a gene encoding organic solvent-tolerant alkaline protease from Pseudomonas aeruginosa strain K. Journal of Basic Microbiology, 50 (2). pp. 143-149. ISSN 0233-111X; ESSN: 1521-4028

Abstract / Synopsis

A gene encoding an organic solvent-stable protease was amplified from Pseudomonas aeruginosa strain K by polymerase chain reaction using consensus primers based on multiple sequence alignment of alkaline and metalloprotease genes from Pseudomonas species. The gene, which consisted of 1440 bp nucleotides and deduced 479 amino acid residues, was successfully expressed in pGEX-4T-1 expression system in the presence of 1.0 mM IPTG, after an incubation of 6 h at 37 degrees C. Under these conditions, the recombinant strain K protease was, subsequently, released into the periplasm of E. coli BL21 (DE3) with an optimum proteolytic activity detected at 1.0112 U/ml. To date, this is the first reported expression of alkaline protease (aprA) with such remarkable property in Escherichia coli.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
DOI Number: 10.1002/jobm.200900133
Publisher: Wiley-VCH Verlag GmbH & Co. KGaA
Keywords: Pseudomonas aeruginosa strain K; Organic solvent-tolerant protease gene; aprA; Expression
Depositing User: Nida Hidayati Ghazali
Date Deposited: 24 Jul 2012 11:18
Last Modified: 26 Sep 2016 12:57
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1002/jobm.200900133
URI: http://psasir.upm.edu.my/id/eprint/15709
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