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Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia.


Citation

Rosli, N. and Mahmood, Maziah and Chan, K. L. and Sreeramanan, S. (2009) Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia. Journal of Forestry Research, 20 (1). pp. 54-58. ISSN 1007-662X

Abstract

A study was conducted to improve 9-methoxycanthin-6-one productivity (potential anti-tumour compound) from callus cultures of Eurycoma longifolia (Tongkat Ali). Several factors affecting 9-methoxycanthin-6-one production in callus cultures such as different medium compositions and physical factors were investigated and analyzed. Results show that a higher production of 9-methoxycanthin-6-one (3.84 mg'g-1 DW (Dry Weight)) is obtained from callus cultured in ¼ MS basal media. At fructose of 2% (w/v), the production of 9-methoxycanthin-6-one (4.59 mg'g-1 DW) is promoted to gain the highest yield, compared to other carbon sources tested. The addition of 2.0-mg'L-1 dicamba also increases 9-methoxycanthin-6-one production (12.3 mg'g-1 DW). Higher production of 9-methoxycanthin-6-one was obtained at pH 5.5 (1.53 mg'g-1 DW). Production of 9-methoxycanthin-6-one (2.34 mg'g-1 DW) in callus cultures is also increased when the medium is added with 1×10-1 μM phenylalanine. This study suggests that the successful production of 9-methoxycanthin-6-one in vitro cultures has a potential in large-scale production using bioreactor technology.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1007/s11676-009-0010-6
Publisher: Springer Verlag
Keywords: Eurycoma longifolia; Callus culture; 9-methoxycanthin-6-one; Dry weight.
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 23 Jul 2013 05:26
Last Modified: 02 Nov 2015 23:59
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s11676-009-0010-6
URI: http://psasir.upm.edu.my/id/eprint/14696
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