Citation
Abdullah Al-Haj, Nagi Ahmed and Shamsudin, Mariana Nor and Abdul Rahim, Raha and Ishak, Zamri
(2008)
Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set.
Research Journal of Biological Sciences, 3 (11).
pp. 1327-1332.
ISSN 1815-8846; ESSN: 1993-6087
Abstract
Although, PCR methods aimed on the detection of genes associated with the pathogenicity of Escherichia coli have been reported, tests allowing the direct identification of this serotype are rare. In this study the Random Amplified Polymorphic DNA (RAPD) fingerprinting technique allowed genetic diversity assessment of 25 E. coli isolates of various sources. A highly significant finding from the DNA fingerprinting is the display of a predominant band at a size of 308 bp when arbitrary OPAE-10 primer was used. After sequencing this fragment primer called secD was designed to be used as PCR primer. secD primer pairs was highly specific to detect all isolates including E. coli O157: H7.
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Official URL or Download Paper: http://medwelljournals.com/journalhome.php?jid=181...
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Medicine and Health Science |
| Publisher: | Medwell Journals |
| Keywords: | Random amplified polymorphic DNA (RAPD); Uropathogenic E. coli (UPEC); Neonatal meningitis-associated E. coli (MNEC); University Putra Malaysia (UPM); Primer; Sequence. |
| Depositing User: | Raja Norazlinda Raja Azenam |
| Date Deposited: | 30 Apr 2014 08:26 |
| Last Modified: | 03 Dec 2015 02:58 |
| URI: | http://psasir.upm.edu.my/id/eprint/13994 |
| Statistic Details: | View Download Statistic |
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