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Antimicrobial susceptibility, plasmid profiles, and RAPD-PCR typing of Acinetobacter bacteria.


Citation

Sadeghifard, Nourkhoda and Ranjbar, Reza and Zaeimi, Javad and Alikhan, Mohammad Yousef and Ghafouryan, Sobhan and Raftari, Mohammad and Abdulamir, Ahmed Sahib and Delpisheh, Ali and Mohebi, Reza and Abu Bakar, Fatimah (2010) Antimicrobial susceptibility, plasmid profiles, and RAPD-PCR typing of Acinetobacter bacteria. Asian Biomedicine, 4 (6). pp. 901-911. ISSN 1905-7415; ESSN: 1875-855X

Abstract

Background: Multiple-drug resistant Acinetobacter have widely spread in the last decades imposing a serious nosocomial source of infection. Nevertheless, little knowledge was gaimed on tracing the development of antibiotic resistance in Acinetobacter species. Objectives: Explore Acinetobacter spp. via antimicrobial susceptibility, plasmid profiles, and random amplified polymorphism DNA polymerase chain reaction (RAPD-PCR) typing.Methods: One hundred twelve Acinetobacter isolates (including 66 A. baumannii and 46 non-Acinetobacter baumannii strains) were obtained from three university hospitals. The source of infection of these isolates included blood, urine, wound, and respiratory tract. Their susceptibilities to 17 antibiotics were tested and then all Acinetobacter isolates were typed by plasmid analysis and RAPD-PCR method.Results: A. baumannii isolates revealed nine different patterns of antibiotic resistance. Of those, non-A. baumannii, were associated with plasmid and RAPD-PCR typings (p 0.05).Conclusion: There is a wide spread of multi-drug resistant Acinetobacter spp., particularly A. baumannii, in the Middle East region that can be traced efficiently by plasmid and genotyping typing of Acinetobacter. More care should be taken for tracing the development of antimicrobial resistance of Acinetobacter using precise molecular typing techniques.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Institute of Bioscience
Publisher: IOS Press
Keywords: Acinetobacter baumannii; Antimicrobial susceptibility; Molecular typing; Plasmid profiles RAPD-PCR.
Depositing User: Khairil Ridzuan Khahirullah
Date Deposited: 03 Apr 2014 03:50
Last Modified: 03 Apr 2014 03:50
URI: http://psasir.upm.edu.my/id/eprint/13160
Statistic Details: View Download Statistic

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