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A unique thermostable and organic solvent tolerant lipase from newly isolated Aneurinibacillus thermoaerophilus strain HZ: physical studies


Citation

Masomian, Malihe and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar and Basri, Mahiran (2010) A unique thermostable and organic solvent tolerant lipase from newly isolated Aneurinibacillus thermoaerophilus strain HZ: physical studies. World Journal of Microbiology and Biotechnology, 26 (9). pp. 1693-1701. ISSN 0959-3993; ESSN: 1573-0972

Abstract

A newly isolated thermophilic bacterium, Aneurinibacillus thermoaerophilus strain HZ, from a hot spring recreational area (Sungai Kelah, Malaysia), showed an extracellular lipase activity. It was identified based on 16S rRNA sequencing, where phylogenetic analysis revealed its homology to Aneurinibacillus thermoaerophilus. The strain produced a lipase that was stable in various organic solvents such as dimethyl sulfoxide, toluene, p-xylene, and hexane. In order to increase lipase production, optimization of physical factors which affected the growth and lipase production was studied. The optimal growth was obtained at 50°C and pH 8.0; while the maximal lipase production was achieved in the logarithmic decline phase at 60°C and pH 7.5 with 7% starting inoculum and 150 rev/min shaking rate for 48 h incubation.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
DOI Number: https://doi.org/10.1007/s11274-010-0347-1
Publisher: Springer
Keywords: Aneurinibacillus thermoaerophilus strain HZ; Thermostable lipase; Optimization of physical factors; Organic solvent tolerant
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 22 Jul 2013 04:27
Last Modified: 17 May 2016 02:51
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s11274-010-0347-1
URI: http://psasir.upm.edu.my/id/eprint/12893
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