Effects of Subcutaneous and Intravenous Recombinant Human Erythropoietin Treatments on Body Iron in Rats

Ahmed Shujaaedin, Hareth Yahya (2009) Effects of Subcutaneous and Intravenous Recombinant Human Erythropoietin Treatments on Body Iron in Rats. Masters thesis, Universiti Putra Malaysia.

[img] PDF
434Kb

Abstract

Recombinant human erythropoietin (rHuEPO) is used widely in clinical practice for correcting anemia related to renal failure, cancer chemotherapy, HIV infection, premature infants, and chronic diseases. Recombinant human erythropoietin is known to affect body iron status that may result in functional iron deficiency (FlO). Functional iron deficiency is one of the major causes of insufficient response to rHuEPO. Thus rHuEPO treatment must be accompanied with iron supplementation to avoid iron metabolism disorder and to maintain optimal erythropoiesis. The aim of this study was to compare the effect of subcutaneous (s.c.) with the intravenous (i.v.) rHuEPO administration on the body iron status in rats after short-term and long-term treatments. For the short-term experiment, 20 Sprague-Dawley rats were divided into four groups of 5 rats each; s.c. rHuEPO group (s.c. 150 IU rHuEPO/kg/day), control group for s.c. rHuEPO (s.c. 0.40 - 0.44 mL 0.9% saline solution/rat/day), i.v. rHuEPO group (i.v. 150 IU rHuEPO/kg/day), and control group for i.v. rHuEPO (i.v. 0.40 - 0.45 mL 0.9% saline solution/rat/day). The duration of the short-term rHuEPO treatments was 7 days. For the longterm experiment 80 Sprague-Dawley rats were divided into four groups of 20 rats each ; s.c. rHuEPO group (s.c. 450 IU rHuEPO/kg/wk), control group for s.c. rHuEPO (s.c. 0.40 - 0.50 mL 0.9% saline solution/rat/wk), i.v. rHuEPO group (i.v. 450 IU rHuEPO/kg/wk), and control group for i.v. rHuEPO (i.v. 0.40 - 0.50 mL 0.9% saline solution/rat/wk). The duration of the long-term rHuEPO treatments was 8 weeks. Blood samples were drawn at the end of the short-term experiment and at wk 2 , wk 6 and wk 8 in long-term experiment. Erythrocyte (RBC) counts, haemoglobin (Hb) concentrations, haematocrit (HCT) and blood smears for reticulocytosis were used to detect activation of erythropoiesis caused by rHu EPO treatment. Serum Iron (SI), transferrin saturation (TS), unsaturated iron binding capacity (UIBC), total iron binding capacity (TIBC), serum ferritin (SF), stainable liver iron (LI), and bone marrow iron (BMI) were analysed to determine body iron status (BIST). Erythr:oc (p<0 .05) higher in all treatment groups compared to the control groups in both short-term and long-term experiments. The blood smears showed increase in reticulocytes in all treatment groups, while no increase in reticulocytes were observed in the control groups in both short-term and long-term experiments. The SI, TS, and BMI were significantly (p<O.05) lower in the treatment groups of short-term and long-term experiments compared to controls. The TIBC was Significantly (p<O.05) higher in the treatment group of short-term but not in long-term experiment while SF and LI showed no significant (p>O.05) difference between groups except in short-term s.c group. The degree of these changes was greater in the i.v. rHuEPO than in the s.c rHuEPO group both for the short-term and long-term experiments. The effect of rHuEPO on iron parameters that suggested increased iron utilization was more apparent in short-term experiment than long-term experiment. In conclusion, this study suggests that among the effects of rHuEPO administration is increasing erythropoiesis through the utilization of serum and storage irons, and that the effect is more pronounced with i.v. than s.c rHuEPO administration particularly in short-term treatment.

Item Type:Thesis (Masters)
Chairman Supervisor:Professor Rasedee Abdullah, PhD
Call Number:FPV 2009 15
Faculty or Institute:Faculty of Veterinary Medicine
ID Code:11540
Deposited By: Nur Kamila Ramli
Deposited On:21 Jun 2011 04:29
Last Modified:21 Jun 2011 04:30

Repository Staff Only: item control page

Document Download Statistics

This item has been downloaded for since 21 Jun 2011 04:29.

View statistics for "Effects of Subcutaneous and Intravenous Recombinant Human Erythropoietin Treatments on Body Iron in Rats"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.